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. Author manuscript; available in PMC: 2023 Feb 2.
Published in final edited form as: Cell Rep. 2022 Jan 11;38(2):110240. doi: 10.1016/j.celrep.2021.110240

Figure 6. Identification of Axin2 as a direct target of Zeb1/YAP transcriptional activation.

Figure 6.

(A) ChIP-PCR analysis showing ZEB1 and YAP binding to the same enhancer site upstream of AXIN2 promoter in MDA-MB-231 cells. GAPDH served as a negative control. Shown are data representative of 3 independent experiments.

(B and C) qRT-PCR analysis of the indicated genes in control and Zeb1-depleted (B; n = 4) or Zeb1-deleted (C; n = 3) basal MECs derived from 12-week-old mice.

(D and E) ZEB1 and YAP1 cooperate to activate the WT (D), but not ZEB1-binding-deficient (E), AXIN2 enhancer. Diagrams of promoter constructs are shown in (D). Black boxes denote E-box motifs. DM, deletion mutant; EV, empty vector; P, promoter.

See also Figure S6.