Figure 7.

Wheat plants tuned into lowered transpiration by CCPs. (A) False-colour thermal images of wheat exposed to ABA and CCPs compared to mock treatment. Half of the plants grown in single pots were mock-treated with the solvent control (left half) or exposed to 100 μM ABA and ABA agonists as indicated (right half). Thermal images of 30-day-old wheat were taken 24 h after administration and two replicates are shown per treatment. (B) The leaf temperature increase of ABA-treated wheat in comparison to mock-treated plants. The leaf temperature difference was averaged from six independent experiments each having three biological replicates (leaf temperature of mock-treated plants, 22.1 ± 0.17°C; 20.7 ± 0.16°C; 21.9 ± 0.19°C; 21.4 ± 0.04°C; 21.7 ± 0.15°C; 21.8 ± 0.17°C). (C) Leaf temperature increase of CCP-treated plants relative to ABA-treated plants set to 100%. The temperature increase at 100 μM ABA was 0.6 ± 0.18°C for CCP1, 0.9 ± 0.19°C for CCP2 and CCP5, 0.6 ± 0.12°C for CCP3 and CCP4, 0.9 ± 0.03°C for CCP6, 0.9 ± 0.10°C for CCP7, and 0.9 ± 0.07°C for CCP8 to CCP9, respectively. The scale bar in (A) indicates 5 cm. (B) **P < 0.01 compared to mock-treated plants. (C) n = 3 biological replicates per treatment, mean ± SEM.