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Yops secreted by Y. enterocolitica W22703. Bacteria were grown at 28°C in a conical flask (seen from the top) containing oxalated brain heart infusion and then transferred to 37°C. The photograph showing the Yop filaments was taken 4 h after the temperature shift. SDS-PAGE of the filaments (right lane) and of Yops precipitated from the supernatant by ammonium sulfate (left lane) is shown on the right. Adapted from reference 229.
