TABLE 5.
Control of Yop release
| Protein | Size (kDa) | Structural featuresa | Role(s) | Localization
|
Relevant similarities | References | |
|---|---|---|---|---|---|---|---|
| Minus Ca2+ | Plus Ca2+ | ||||||
| YopN | 32.6 | Coiled-coil structures (aa 62–108 and aa 248–272) | Element of the putative plug closing the secretion channel; associated with TyeA | Secreted, cytosolic | Cytosolic, surface associated | MxiC (Shigella spp.); InvE (Salmonella spp.); PopN (P. aeruginosa); HrpI (E. amylovora); CopN (C. psittaci) | 6, 38, 99, 117, 165, 172, 379 |
| TyeA | 10.8 | Element of the putative plug closing the secretion channel; binds to the second coiled-coil domain of YopN; binds to YopD; required for translocation of YopE and YopH but not YopM, YopO, YopP, YopT | Surface associated, cytosolic | Surface associated, cytosolic | Pcr1 (P. aeruginosa); SsaL (Salmonella spp.) | 99, 154, 170, 172, 360, 379 | |
| LcrG | 11.0 | Forms dimers; heparin-binding domain | Involved in control of secretion; required for efficient delivery of all Yop effectors; binds to heparan proteoglycans | Mainly cytosolic | Mainly cytosolic | PcrG (P. aeruginosa) | 48, 246a, 247, 294, 295, 311, 312, 379 |
a
aa, amino acids.