表 1.

RT-qPCR不同基因的引物序列

The primer sequences of different genes for RT-qPCR

Gene	Primer sequences
TNF-α, tumor necrosis factor-α; IL-1β, interleukin-1β; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
TNF-α	Forward: 5′-CTTCTCATTCCTGCTCGTG-3′
	Reverse: 5′-TTTGGGAACTTCTCCTCCT-3′
IL-1β	Forward: 5′-TTCATCTTTGAAGAAGAGCCC-3′
	Reverse: 5′-CTGTCTAATGGGAACATCACAC-3′
GAPDH	Forward: 5′-AACTCCCATTCTTCCACCT-3′
	Reverse: 5′-TTGTCATACCAGGAAATGAGC-3′