Figure 1.

Characteristics of peach peel extract (PE). (a) Protein pattern of peach peel extract (PE) was determined via SDS-PAGE and Coomassie staining, natural Pru p 3 (nPru p 3; P) was used as reference; (b) nPru p 3 or PE were subjected to SDS-PAGE followed by immunoblot analysis. To determine allergen reactivity, nsLTP-reactive rabbit serum was used and inhibition of antibody binding was performed with increasing dosages of nPru p 3 or BSA as control. Negative control (N) or secondary antibody control (S) were incubated with either buffer or only secondary antibody.