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. 2023 Feb 2;9:7. doi: 10.1038/s41522-023-00375-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Representative confocal flow cell images stained with SYTO62 (pink) and the Pel-specific lectin WFL-FITC (green) to detect biomass and Pel, respectively. Scale bars = 100 μm. b Dot blot of crude cell-associated and secreted Pel fractions of the indicated strains. Pel was detected using WFL-HRP. P_BAD pel, PAO1 ∆wspF ∆psl P_BADpel.