TABLE 3.
Examples of uORF-containing leaders in S. cerevisiae
| Gene | Length of major 5′UTR (nt)a | No. and size of uORFs (no. of codons) | Product (reference) |
|---|---|---|---|
| CBS1 | 101 | uORF (4) | PET gene involved in the 5′ end processing of the cytochrome b (57) |
| CLN3 | 864 | uORF (1) | G1 cyclin (441) |
| CPA1 | 244 | uORF (26) | Small subunit of cytosolic carbamoyl phosphate synthetase (406) |
| DCD1 | 33 | uORF (4) | dCMP deaminase (366) |
| GCN4 | 591 | uORF1 (4), uORF2 (3), uORF3 (4), uORF4 (4) | Transcriptional activator of amino acid biosynthetic pathway (391) |
| HAP4 | ∼280 | uORF1 (10), uORF2 (4) | Subunit of transcriptional activator complex binding CCAAT (156) |
| HOL1 | ∼385 | uORF (6) | Major facilitator family (drug resistance subfamily) of putative transport proteins (598) |
| LEU4 | 85 | uORF1 (13) | α-Isopropylmalate synthase (cytoplasmic) (43) |
| PET111 | 459 | uORF1 (6), uORF2 (31), uORF3 (11), uORF4 (30) | Mitochondrial translational activator (528) |
| PPR1 | 50 | uORF (6) | Regulatory protein controlling transcription of two genes in pyrimidine biosynthesis pathway (278) |
| SCHO9 | ∼600 | uORF (55) | Protein kinase that positively regulates the progression of yeast through G1 phase (56) |
| SCO1 | ∼150 | uORF (3) | PET gene involved in the accumulation of cytochrome c oxidase subunits I and II (304) |
| TIF4631 | 295 | uORF1 (12), uORF2 (20), uORF3 (16), uORF4 (8), uORF5 (12), uORF6 (22) | Translation initiator factor p150 (180) |
| YAP1 | 164 | uORF (7) | Stress-related transcription factor (386) |
| YAP2 | 157 | uORF1 (6), uORF2 (23) | Stress-related transcription factor (62) |
a
nt, nucleotides.