Figure 4.
IFN-γ secretion in PBMCs, BM, spleen and pancreas of NACL or ZOL-injected and tooth-extracted hu-BLT mice. Hu-BLT mice were administered with either 0.9% NACL or ZOL (500 µg/kg) via IV followed by maxillary left first molar extraction as described in Materials and Methods section. Two (A) or four weeks (B) after tooth extraction, mice were euthanized and tissues were harvested to obtain single cell suspension. PBMCs (n=4), BM (n=4), splenocytes (n=4), and pancreas (n=3) of hu-BLT mice were cultured (2 × 106 cells/2ml) with IL-2 (1000 U/ml) for three days, after which the supernatants were harvested and the levels of IFN-γ were determined using specific ELISA. IFN-γ secretion per human CD45+ cell was determined using CD45 percentages obtained by flow cytometric analysis. IFN-γ per one NK cell was determined using CD16+CD56+ percentages obtained by flow cytometric analysis (A, B). ****(p value<0.0001), ***(p value<0.001), **(p value 0.001-0.01), *(p value 0.01-0.05), ns (no significance).