Figure 5.

NK cell-mediated cytotoxicity in PBMCs, BM, spleen and pancreas of NACL or ZOL-injected and tooth-extracted hu-BLT mice. Hu-BLT mice were administered with either 0.9% NACL or ZOL (500 µg/kg) via IV followed by maxillary left first molar extraction as described in Materials and Methods section. Two (A) or four weeks (B) after tooth extraction, mice were euthanized and tissues were harvested to obtain single cell suspension. PBMCs (n=4), BM (n=4), splenocytes (n=4), and pancreas (n=3) of hu-BLT mice were cultured (2 × 10⁶ cells/2ml) with IL-2 (1000 U/ml) for three days, after which the cells were used as effector cells in standard 4-hour ⁵¹Cr release assay against human OSCSCs tumors. Lytic units (LU) 30/10⁶ cells were determined using inverse number of effector cells required to lyse 30% of OSCSCs × 100. LUs per 1% NK cell were determined using CD16+CD56+ percentages obtained by flow cytometric analysis (A, B). ***(p value<0.001), **(p value 0.001-0.01), *(p value 0.01-0.05), ns (no significance).