Figure 6.

IFN-γ secretion and NK-cell mediated cytotoxicity in NACL and denosumab-injected hu-BLT mice. Hu-BLT mice were administered with either 0.9% NACL or denosumab (120 mg/mice) via IV as described in Materials and Methods section. Four weeks after injections, mice were euthanized and tissues were harvested to obtain single cell suspension. PBMCs (n=2), BM cells (n=2), splenocytes (n=2), pancreas (n=2), and gingiva (n=3) of hu-BLT mice were cultured (2 × 10⁶ cells/2ml) with IL-2 (1000 U/ml) for three days, after which the supernatants were harvested and the levels of IFN-γ were determined using specific ELISA (A). PBMCs (n=2), BM cells (n=2), and splenocytes (n=2) were used as effector cells in standard 4-hour ⁵¹Cr release assay against human OSCSCs tumors. Lytic units (LU) 30/10⁶ cells were determined using inverse number of effector cells required to lyse 30% of OSCSCs × 100 (B). LUs per 1% NK cells were determined using CD16+CD56+ percentages obtained by flow cytometric analysis (n=2) (C). ***(p value 0.0001-0.001), **(p value 0.001-0.01), *(p value 0.01-0.05), ns (no significance).