Table 5.
Percentages of immune cells in PBMCs, BM, splenocytes and pancreas of NACL or ZOL-injected and tooth-extracted hu-BLT mice.
| Hu-BLT week 4 (n=4) | Peripheral blood mononuclear cells | Bone marrow | Spleen | Pancreas | ||||
|---|---|---|---|---|---|---|---|---|
| NACL | ZOL | NACL | ZOL | NACL | ZOL | NACL | ZOL | |
| % CD16+CD56+ | 6 | 8.3 | 2.7 | 5.1 | 2.3 | 4.04 | 2.3 | 5.2 |
| % CD3+ | 68 | 55.3 | 16.5 | 30.5 | 28 | 51.3 | 40.5 | 60.3 |
| % CD3+CD4+ | 63 | 78.5 | 66 | 54 | 74 | 85 | 20 | 25 |
| % CD3+CD8+ | 37 | 21.5 | 35 | 46 | 26.3 | 15 | 80 | 75 |
Hu-BLT mice were administered with either 0.9% NACL or ZOL (500 µg/kg) via IV followed by maxillary left first molar extraction as described in Materials and Methods section. Four weeks after tooth extraction, mice were euthanized and tissues were harvested to obtain single cell suspension. Immune cell composition was determined in PBMCs (n=4), BM (n=4), splenocytes (n=4), and pancreas (n=3) of hu-BLT mice using flow cytometric analysis.