| D | ||||
|---|---|---|---|---|
| IFN-γ/NK Cell Week 4 |
BLT (NACL+ tooth extraction) |
BLT (ZOL + tooth extraction) |
Fold change ZOL/NACL |
p values row 2 vs. row 3 |
| Bone marrow (n=4) | 1.1 +/- 0.33 | 1.9 +/- 1.1 | 1.7 | ns |
| Spleen (n=4) | 7.5 +/- 0.7 | 11 +/- 3 | 1.5 | * |
| Gingiva (n=2) | 0.5 +/- 0.14 | 0.04 +/- 0.006 | 0.08 | *** |
| Peripheral blood mononuclear cells(n=4) | 113 +/- 6 | 104 +/- 6 | 0.9 | ns |
| Pancreas (n=3) | 0.2 +/- 0.023 | 0.4 +/- 0.1 | 2 | * |
Hu-BLT mice were administered with either 0.9% NACL or ZOL (500 µg/kg) via IV followed by maxillary left first molar extraction as described in Materials and Methods section. Two (A, C) or four (B, D) weeks after tooth extraction, mice were euthanized and tissues were harvested to obtain single cell suspension. BM (n=4), splenocytes (n=4), PBMCs (n=4), oral gingiva (n=2), and pancreas (n=3) of hu-BLT mice were cultured (2 × 106 cells/2ml) with IL-2 (1000 U/ml) for three days, after which the supernatants were harvested and the levels of IFN-γ were determined using specific ELISA. Levels of IFN-γ secreted was determined based on one million human CD45+ immune cells (A, B) or based on one NK cell (C, D). ****(p value <0.0001), ***(p value <0.001), **(p value 0.001-0.01), *(p value 0.01-0.05) ns, no-significance.