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. 2023 Feb 2;13:1886. doi: 10.1038/s41598-023-28862-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Ordination plot of principal components 1 and 2 for confirmed metabolites detected in HILIC (left) and C18 (right) by infant age in days. R²- and P- values calculated using Permutational Multivariate Analysis of Variance, with age in days as the explanatory variable. Points are colored by visit, with the age in days corresponding with each visit as follows: 01 m (25–46 days), 06 m (164–219 days), 12 m (351–429 days), 18 m (517–582 days), and 24 m (709–916 days).