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. 2023 Feb 2;14:558. doi: 10.1038/s41467-023-36315-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Co-immunostaining of NR5A2 (green), insulin (Red), GCG (White) and DAPI (blue) in db/+ (left), db/db (middle), or β-Aldh1a3 KO_db/db (right) pancreata. Scale bars: 100 μM. B Ratio of NR5A2-positive cells in INS+/GCG- β-cells. All data are expressed as means ± SEM. N = 14, 23 or 16 islets from 4 biologically independent samples per group. One-way ANOVA with multiple comparison test was used for statistical analysis. C Co-immunostaining of KI67 (green) and INS (white) in db/db or β-Aldh1a3 KO_db/db pancreata. Scale bars: 100 μM. D Ratio of KI67-positive cells to total islet cells (left) or INS+/GCG- cells (right). All data are expressed as means ± SEM. N = 18 or 24 islets from 4 biologically independent samples per group. Two-tailed paired t-test was used for statistical analysis. Source data are provided as a Source Data file.