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Propionate is an additional carbon source which is supplied as a cosubstrate for the synthesis of P(3HB-3HV) in recombinant E. coli. Several pathways have been shown to be involved in the uptake of propionate and are important in defining the optimal genotype for P(3HB-3HV) production strains. Both the acetoacetate degradation pathway (the Ato system) and the acetate secretion pathway (Ack/Pta) have been identified as contributing to propionate transport.
