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Biosynthesis of P(3HB-4HB) in recombinant E. coli by using heterologous genes from Clostridium kluyveri. The 4HB monomer in the synthesis of P(3HB-4HB) is derived from succinate. Succinate is converted to 4HB-CoA by enzymes that are encoded by genes from the gram-positive, strictly anaerobic C. kluyveri microbe.
