Table 2.
Assignment of tryptic digested peptides of Nostoc_MUM004 to protein families by Q-TOF-LC–MS/MS accessed on 13 March 2020 using Uniprot Nostoc NIES-2111, Swissprot + TrEMBL
| Protein name | Accession number | Peptide sequence | m/z | RT | Query cover (%) | E value |
|---|---|---|---|---|---|---|
| AAA family ATPase [Nostoc sp. NIES-2111] | WP_096680967.1 | ETLQFSK | 426.7574 | 21.26 | 100 | 2.7 |
| DUF4114 domain-containing protein [Nostoc sp. NIES-2111] | WP_096683138.1 | IESSTSK | 376.244 | 20.48 | 100 | 4.5 |
| ferredoxin-NADP + oxidoreductase [Desmonostoc sp. PCC 7906] | BAG69182.1 | AVRQLQK | 421.7616 | 17.07 | 71 | 0.23 |
| sensor histidine kinase [Nostoc sp. NIES-2111] | WP_096679804.1 | LNTIHSK | 271.53 | 17.07 | 100 | 9.9 |
| insulinase family protein [Nostoc sp. PA-18-2419] | WP_138499051.1 | ALQAEIK | 332.9 | 19.93 | 100 | 1.6 |
| CHAT domain-containing protein [Nostoc sp. NIES-2111] | WP_096681907.1 | SLSLLGGTVV | 473.346 | 43.71 | 100 | 0.012 |
| nitrate reductase [Nostoc linckia] | WP_099069194.1 | FHTPDGRAR | 352.9 | 28.22 | 100 | 0.003 |
| family 10 glycosylhydrolase [Nostoc piscinale] | WP_062289469.1 | ESAIARNGK | 473.346 | 43.71 | 100 | 0.028 |
| MULTISPECIES: 16S rRNA (cytosine(967)-C(5))-methyltransferase [unclassified Nostoc | WP_067768222.1 | QYIRSDEASP | 292.1898 | 20.5 | 100 | 4.00E-04 |
| serine/threonine protein kinase [Nostoc sp. NIES-2111] | WP_096678107.1 | KNIVWLLILALL | 353.0538 | 45.88 | 100 | 2.00E-06 |
| GAF domain-containing protein [Nostoc sp. NIES-2111] | WP_067770071.1 | LPSLLGLHFPAD | 427.2707 | 21.2 | 100 | 6.00E-09 |
| type I-D CRISPR-associated helicase Cas3’ [Nostoc sp. NIES-2111] | WP_096680030.1 | HAGFSAYQAFV | 599.2462 | 30.46 | 100 | 6.00E-05 |
| PAS domain S-box protein [Nostoc sp. NIES-2111] | BAY37603.1 | LAELELLQQK | 296.945 | 21.24 | 100 | 1.00E-03 |
| type I restriction-modification system endonuclease [Nostoc cycadae] | WP_103124733.1 | QDAGIEVGR | 236.9427 | 23.05 | 100 | 2.20E-02 |
| MULTISPECIES: HAMP domain-containing protein [unclassified Nostoc] | WP_067768065.1 | FTSAGGK | 223.1751 | 21.51 | 100 | 4.70E + 00 |
| type I restriction-modification system endonuclease [Nostoc cycadae] | WP_103124733.1 | DFNDYVSK | 494.3077 | 22.92 | 100 | 2.20E-02 |
| 30S ribosomal protein S4 [Nostoc sp. PA-18-2419] | WP_138504283.1 | AYPPGQHGQNR | 306.848 | 29.06 | 100 | 2.00E-05 |
| ABC transporter ATP-binding protein [Nostoc sp. NIES-3756] | WP_067768587.1 | YEVWELIRQLK | 370.0337 | 48.66 | 100 | 3.00E-06 |
| DUF3854 domain-containing protein [Nostoc sp. NIES-2111] | WP_096682741.1 | IAQNIYSK | 234.9618 | 12.49 | 100 | 4.40E-02 |
| SDR family NAD(P)-dependent oxidoreductase [Nostoc sp. NIES-2111] | WP_096681600.1 | ATLAAIAATGSK | 537.8521 | 43.99 | 100 | 3.00E-04 |
| alkanesulfonate monooxygenase [Nostoc sp. NIES-2111] | BAY36072.1 | ELQSSKTVER | 294.864 | 19.25 | 100 | 1.00E-03 |
| polynucleotide adenylyltransferase region [Nostoc linckia NIES-25] | BAY77126.1 | TPALQTR | 262.8481 | 17.43 | 100 | 1.20E + 00 |
| M1 family metallopeptidase [Nostoc sp. NIES-2111] | WP_096681268.1 | LSAIRALGK | 232.9722 | 2.76 | 100 | 5.50E-02 |
| NAD(P)/FAD-dependent oxidoreductase [Nostoc sp. ‘Lobaria pulmonaria (5183) cyanobiont’] | WP_104906479.1 | VNPAQLR | 200.1826 | 31.6 | 100 | 8.20E-02 |
| ATP-dependent DNA helicase RecG [Nostoc sp. ‘Peltigera malacea cyanobiont’ DB3992] | WP_099100676.1 | DLLFYYPR | 543.9307 | 48.59 | 100 | 5.00E-03 |
| hypothetical protein [Nostoc sp. NIES-2111] | WP_096678986.1 | HFGTPIR | 414.1471 | 28.88 | 100 | 2.90E-01 |
| ParB/RepB/Spo0J family partition protein [Nostoc sp. NIES-2111] | WP_096683715.1 | TASSTSNSESNNYK | 373.1037 | 47.28 | 100 | 3.00E-07 |
| hypothetical protein [Nostoc sp. NIES-2111] | WP_096678378.1 | TATAECWGR | 263.9357 | 17.77 | 100 | 2.00E-03 |
| hypothetical protein [Nostoc sp. NIES-2111] | WP_096683493.1 | DIGCYGIVFR | 300.8999 | 36.14 | 100 | 9.00E-05 |