FIG. 14.

Minus-strand synthesis reactions of normal and mutant procapsids (61). Procapsids were prepared from cells producing proteins containing the mutation of ERA12 that results in a carrier-state infection where segment S is missing from the procapsids. Nonradioactive plus-strand RNA was added alone (s, m, l) or in combinations (sm, sl, ml, sml) to procapsids under conditions for minus-strand synthesis with radioactive nucleotides. Note that procapsids containing mutant P1 do not need segment S for minus-strand synthesis of segments M and L.