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. 2022 Dec 2;83(3):363–373. doi: 10.1158/0008-5472.CAN-22-0074

Figure 3.

Figure 3. Gene-based exploration of the IMMUcan scDB using CXCL13, a predictive biomarker for immunotherapy response. A, Heatmap of CXCL13 expression across datasets (y-axis) and annotation minor cell types (x-axis). B and C, UMAP plots of BCC_BIA_10X_GSE123813 dataset colored by cell type (minor annotation, B) and CXCL13 expression (C). D and E, Co-expression plot of CXCL13 and PDCD1 (PD1), cells are colored according to the minor annotation displaying all cell types (D) and only exhausted CD8+ T cells (T CD8 ex) and Tfh (E). The legend indicates the cell type with the number of expressing cells and the Pearson correlation coefficient in brackets. F, Venn diagram showing the co-expression of CXCL13 and PDCD1 by T CD8 ex (top) and Tfh (bottom). The P value of a hypergeometric test is shown in the top-right corner of each plot, a pie chart representing the proportion of expressing cells for one of the two genes is in the bottom-right corner of each plot.

Gene-based exploration of the IMMUcan scDB using CXCL13, a predictive biomarker for immunotherapy response. A, Heatmap of CXCL13 expression across datasets (y-axis) and annotation minor cell types (x-axis). B and C, UMAP plots of BCC_BIA_10X_GSE123813 dataset colored by cell type (minor annotation; B) and CXCL13 expression (C). D and E, Coexpression plot of CXCL13 and PDCD1 (PD1), cells are colored according to the minor annotation displaying all cell types (D) and only exhausted CD8+ T cells (T CD8 ex) and Tfh (E). The legend indicates the cell type with the number of expressing cells and the Pearson correlation coefficient in brackets. F, Venn diagram showing the coexpression of CXCL13 and PDCD1 by T CD8 ex (top) and Tfh (bottom). The P value of a hypergeometric test is shown in the top-right corner of each plot; a pie chart representing the proportion of expressing cells for one of the two genes is in the bottom-right corner of each plot.