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. 2023 Jan 27;22:15330338221145994. doi: 10.1177/15330338221145994

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© The Author(s) 2023

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — Serine deprivation increased the expression of PHGDH in colon cancer. (A) Quantification of PHGDH mRNA levels in colon cancer cells (SW620, SW480, HT29 and HCT116) cultured in basic and serine-deprived medium for 24 h. Error bars represent mean ± SD (n = 3). *p < 0.05. (B) Immunoblotting analysis of PHGDH protein expression in colon cancer cells (SW620, SW480, HT29 and HCT116) cultured in basic and serine-deprived medium for 24 h. (C) and (d) SW620 was cultured in basic and serine-deprived medium and harvested at the indicated hours; mRNA levels and protein levels (D) of PHGDH. Error bars represent mean ± SD (n = 3), *p < 0.05. (E) Immunoblotting analysis of ATF4 protein expression in SW620 cells cultured in basic and serine-deprived medium for 12 h and harvested at the indicated hours. (F) and (G) SW620 transfected with indicated si-ATF4 (1#, 2#) and cultured in basic and serine-deprived medium; the mRNA expressions of ATF4 and PHGDH, and protein levels of PHGDH. Error bars represent mean ± SD (n = 3), *p < 0.05.