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. 2023 Jan 27;22:15330338221145994. doi: 10.1177/15330338221145994

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© The Author(s) 2023

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — Post-translational modification changes of PHGDH protein under serine deprivation. (A) 293T cells transfected with Flag-PHGDH plasmid in complete medium and serine withdrawal medium, respectively, and the protein samples collected after Immunoprecipitation were electrophoresed by SDS-PAGE and the protein distribution maps stained with Kaomasbrilliant blue. The target protein (∼57KD) was collected and analyzed by HPLC-MS. The figure on the right shows the primary mass spectrum of the target protein. (B) and (C) Under complete medium (SW620-Con), PHGDH undergone acetylation modification, with specific sites K289 and K364. (D) and (E) Under serine starvation conditions (SW620-Ser), the acetylation site at position K364 and K21.