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. 2023 Feb 1;11(2):e005545. doi: 10.1136/jitc-2022-005545

Figure 2.

Figure 2

Cell–cell communication and spatial neighborhoods associated with response to ICI in NSCLC. (A) Prevalence of CXCL13+ T cells, CXCL13+ Th and CXCL13+ Tc across non-responders and responders as a proportion of T cells. CXCL13+ T cells – non-responders versus responders: *p=0.021645. CXCL13+ Th non-responders versus responders: *p=0.021645. (B) Pie chart indicating the relative proportion of cell types expressing CXCL13 (n=11). (C) Heatmap indicating significant pairwise cell-type interaction (red) or avoidance (blue) summarized across the two-sided permutation tests on individual images (n=11 images; 1000 permutations each). (D) Heatmap of seven cellular neighborhoods discovered in 11 patients with NSCLC. (E) Number of cells per cellular neighborhood in non-responders and responders to ICI (CN2 – non-responders vs responders *p=0.0303). Median±IQR. Statistical analysis (A, E: Mann-Whitney test). Cl Mo, classical monocyte; ICI, immune checkpoint inhibitor; Int Mo, intermediate monocyte; M1-like MAC, M1-like macrophage; M2-like MAC, M2-like macrophage; NK cell, natural killer cell; NSCLC, non-small cell lung cancer; Tc, cytotoxic T cell; Th, helper T cell; Treg, regulatory T cell.