Figure 3.

Relative content of isoprenoids from purified plastids and transcripts expression of key genes of green and mature berry skin of red grape berry cv. “Vinhão”, involved in the biosynthesis of isoprenoids. (A) Chlorophyll biosynthesis pathway. (B) Relative expression of key genes involved in the biosynthesis of chlorophylls. (C) Isoprenoid biosynthesis pathway with the ratio of metabolites (log2 green/mature) identified in purified plastids from the skin of grape berries and leaves at green (E-L 34) and mature (E-L 38) stages of development. (D) Relative expression of key genes involved in isoprenoid biosynthesis. Gray metabolite and blue gene names indicate measurements from the present study. Asterisks indicate statistical significance between mature (E-L 38) and green (E-L 34) stages following the Student’s t-test: *P ≤ 0.05; **P ≤ 0.01; and ***P ≤ 0.001.