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. 2023 Feb 2;21:29. doi: 10.1186/s12964-022-01022-y

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — NEDD4 regulates SF3A2 ubiquitination indirectly inhibiting NLRP3 inflammasome activation. A, B HEK293T cells were transfected with Myc-SF3A2 and HA-UB, together with the empty control vector or Flag-NEDD4 expression vector. Cell lysates were immunoprecipitated with anti-RIP1 antibody after treatment with or without LPS and Nig stimulation. C CMECs transfected with GFP- and RFC-NLRP3 were fixed and incubated with a secondary antibody conjugated to Alexa Fluor 594 and Alexa Fluor 488. Colocalization between NEDD4 and NLRP3 was examined by fluorescence microscope