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. 2023 Feb 3;20:11. doi: 10.1186/s12987-023-00411-w

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Endothelial tPA controls NVC. A Schematic representation of the generation of VE-Cadherin Cre/tPA Lox mice. The cadherin 5 promoter was used to drive expression of Cre recombinase in the vascular endothelium. LoxP sites were flanked in the exon 3 of tPA gene. This configuration carries out the deletion of endothelial tPA gene while conserving tPA in other cells. B Colormap corresponds to the activation map related ∆CBF changes during whisker stimulations of VECad-Cre^ΔtPA mice and their wild type littermates. Maps were obtained by subtraction of stimulation frames and resting frames. Warm colours indicate an elevation of CBF during whisker stimulations. C Time course of % CBF increase (mean ± SEM) during whisker stimulations () of VECad-Cre^ΔtPA () and their littermates (). D Box plots show the variations of % CBF increase from baseline during whisker stimulation of VECad-Cre^ΔtPA mice and their littermates. Box plots with medians, 1st and 3rd quartiles, min and max with values for each mouse. *p < 0.05 from VECad-Cre^WT, Mann–Whitney test, n = 14/17 VECad-Cre^WT/ VECad-Cre^ΔtPA

Inline graphic — Endothelial tPA controls NVC. A Schematic representation of the generation of VE-Cadherin Cre/tPA Lox mice. The cadherin 5 promoter was used to drive expression of Cre recombinase in the vascular endothelium. LoxP sites were flanked in the exon 3 of tPA gene. This configuration carries out the deletion of endothelial tPA gene while conserving tPA in other cells. B Colormap corresponds to the activation map related ∆CBF changes during whisker stimulations of VECad-Cre^ΔtPA mice and their wild type littermates. Maps were obtained by subtraction of stimulation frames and resting frames. Warm colours indicate an elevation of CBF during whisker stimulations. C Time course of % CBF increase (mean ± SEM) during whisker stimulations () of VECad-Cre^ΔtPA () and their littermates (). D Box plots show the variations of % CBF increase from baseline during whisker stimulation of VECad-Cre^ΔtPA mice and their littermates. Box plots with medians, 1st and 3rd quartiles, min and max with values for each mouse. *p < 0.05 from VECad-Cre^WT, Mann–Whitney test, n = 14/17 VECad-Cre^WT/ VECad-Cre^ΔtPA