Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Feb 3;20:11. doi: 10.1186/s12987-023-00411-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 7 — tPA is present in brain and liver endothelial cells. A Epifluorescence images of brain cortex and liver slices from C57BL/6 adult mice (Scale bar = 50 µm, zooms = 10 µm) revealing tPA (red), CD31 (green) and cells nuclei (DAPI, blue). tPA was observed in endothelial cells (arrow) in both the brain cortex and liver. Lumen of vessels was represented by stars. B Hepatocytes do not express tPA, but liver endothelial cells do. Representative photomicrographs of liver tissue sections subjected to in situ hybridization to reveal plat mRNA (mRNA encoding for the gene tPA; green), phalloïdine (hepatocyte marker; grey) and CD31 (red) staining; scale bar 100 µm and 50 µm in the magnified inserts. Images were digitally captured using a camera (CoolSNAP; Photometrics) and/or with an inverted confocal microscope (SP5, Leica). Images were visualized respectively with Metavue 5.0 software (Molecular Devices, USA) and LAS AF lite software (LEICA)