Fig. 3.

The effects of NRXN1 downregulation on neurite outgrowth in primary rat neurons. A Representative immune-stained images for microtubule association protein-2 (MAP 2) in primary cultured cortical neurons were shown. Scale bar, 50 μm. Wild type (WT), Sh-Nc, Sh-NRXN1. B Quantification of total neurite F (2, 87) = 7.05, P = 0.001. C Quantification of the length of longest neurite F (2, 87) = 4.12, P = 0.019. D Statistics on the number of primary neurites F (2, 87) = 1.36, P = 0.261. E The number of branch points F (2, 87) = 3.29, P = 0.042. The neurite length was determined by ImageJ. These data were presented as mean ± SD of three independent experiments. NS > 0.05, *P < 0.05, **P < 0.01 versus WT group. #P < 0.05, ##P < 0.01 versus Sh-Nc group