Table 2.

Mass and approximate number of particles for in vivo studies with Al-based NPs

	Alum	DUT-4	DUT-5	MIL-53 (Al)	MIL-101 NH2 (Al)
In vivo NP mass dosage (equal mass studies)	100 µg/mouse	100 µg/mouse	100 µg/mouse	100 µg/mouse	100 µg/mouse
In vivo NP approx. number dosage (equal mass studies)	2.43 × 107 particles/mouse	1.36 × 1010 particles/mouse	1.68 × 109 particles/mouse	1.71 × 107 particles/mouse	1.29 × 109 particles/mouse
In vivo NP mass dosage (equal Al studies)	25 µg/mouse	N/A	50 µg/mouse	N/A	N/A
In vivo NP approx. number dosage (equal Al studies)	6.09 × 106 particles/mouse	N/A	8.42 × 109 particles/mouse	N/A	N/A

All in vivo studies (listed in Table 1) were “equal mass studies” with the exception of the “equal Al studies” described in this sub-section. Calculations for approximation of particle numbers can be found in the SI