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. 2022 May 20;1(3):pgac063. doi: 10.1093/pnasnexus/pgac063

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© The Author(s) 2022. Published by Oxford University Press on behalf of the National Academy of Sciences.

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Fig. 1. — Mammalian SLC46A3 shares a lysosomal-sorting motif in its C-terminal region. (A) Predicted membrane topology of SLC46A3 by TMHMM (http://www.cbs.dtu.dk/services/TMHMM/) and sequence alignment of its C-terminal region between mammalian species. The sequences predicted as tyrosine-based lysosomal-sorting motifs are shown with red color characters. Human dC is a C-terminal amino acid sequence deleted in SLC46A3 mutants in this study. (B) and (C) Immunofluorescence staining of FLAG-tagged SLC46A3 (green) in transfected cells by anti-FLAG antibody. HEK293T cells were transfected with 3 × FLAG-tagged wild-type human SLC46A3 (B) or C-terminal deleted mutant human SLC46A3 (SLC46A3 dC) (C) and mCherry or mKate2-tagged organelle marker protein (red). The results were replicated in three independent experiments. Blue color, nucleus. Scale bar, 10 μm. PTS1, peroxisomal targeting signal 1; MTS, mitochondrial targeting sequence; and B4GALT (NT), N-terminal 81-amino acid human beta-1,4-galactosyltransferase.