Fig. 3.

Steroid conjugates and bile acids strongly suppress SLC46A3 dC-mediated estrone 3-sulfate transport. Effect of various compounds on the uptake of [³H]estrone 3-sulfate (10 nM) in MDCKII/SLC46A3 dC cells. Uptake was performed at 37°C, pH 5.0, for 1 min in the absence or presence of inhibitors (20 μM for cholesterol, dehydroepiandrosterone, estrone, pregnenolone, 25-hydroxyvitamin D₃, 25-hydroxyvitamin D₃ glucuronide, 25-hydroxyvitamin D₃ sulfate, hemin, and bilirubin or 200 μM for the other compounds). Data are presented as the mean ± SEM from two biologically independent experiments. P-values are indicated in the different panels (one-way ANOVA followed by Dunnett's multiple comparison).