Table 1.
Kinetic parameters of SLC46A3 dC-mediated steroid conjugates uptake, and predicted LogP and LogD values of steroid conjugates and bile acids.
| Compounds | Km (μM) | Vmax (nmol/min/mg protein) | LogP | LogD5.0 |
|---|---|---|---|---|
| Dehydroepiandrosterone sulfate | 5.6 ± 1.1 | 1.51 ± 0.08 | 3.42 | 1.04 |
| Estrone 3-sulfate | 33.3 ± 2.7 | 1.46 ± 0.04 | 3.83 | 1.46 |
| 25-Hydroxyvitamin D3 sulfate | 0.2 ± 0.1 | 0.27 ± 0.02 | 4.30 | 3.33 |
| Pregnenolone sulfate | 1.2 ± 0.4 | 0.79 ± 0.07 | 3.64 | 1.26 |
| Cholate | 26.7 ± 3.3 | 2.32 ± 0.09 | 2.48 | 1.84 |
| Glycocholate | 14.6 ± 2.7 | 1.31 ± 0.07 | 1.38 | 0.12 |
| Taurocholate | 11.7 ± 2.3 | 0.78 ± 0.04 | −0.24 | −1.46 |
The experiments were performed with two biological replicates to calculate the mean value of Km and Vmax for each compound. Data are presented as the mean ± SEM. (n = 4). Predicted LogP and LogD5.0 values of each compound were calculated with the online platform Chemicalize developed by ChemAxon (http://chemicalize.com).