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. 2020 Feb 4;19(1):194–202. doi: 10.5114/aoms.2020.92830

Figure 3.

Figure 3

MTT-cellular metabolic activity assay for fibroblast cell cultures with different hemostat exposure times. Data are normalized to controls. Human stromal fibroblasts were exposed to hemostats placed in their medium for 5–10 (A), 30 (B), or 60 minutes (min) (C) or continuously over 24 hours (h) (D). Cell metabolic activity was analyzed using an MTT assay after 3, 6 and 24 h. Reduced cellular metabolism was observed for both ORC and ONRC (vs. GELA and controls) at 5–10 and 60 min of hemostat exposure after 3 h (A and C). ONRC showed reduced cellular metabolism vs. control, GELA and ORC after 5–10, 30 and 60 min hemostat exposure after 3 h (A–C). This effect was maintained with continuous hemostat exposure for 24 h (D). Two-way ANOVA with 2-stage step-up method of Benjamini, Krieger and Yekutieli was applied (q = 0.05). No difference in cellular metabolism was found for GELA vs. control at any given exposure time. *p < 0.05 ORC vs. GELA; **p < 0.05 ORC vs. control and GELA; ***p < 0.05 ONRC vs. control, GELA and ORC (n = 8–12/group)