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. 2022 Dec 9;119(50):e2217542119. doi: 10.1073/pnas.2217542119

Fig. 2.

Fig. 2.

BRCA1 BRCT promotes ds-rRNA. (A) 5.8S rRNA per ng RNA from UWB1 cells with or without ectopic expression of WT or S1655A BRCA1 BRCT was quantified using RT-qPCR. Y-axis represents fold difference relative to the UWB1 cells transfected with vector alone. (B and C) Intact 5.8S rRNA (B) and ACTB (C) post RNase A treatment normalized to total 5.8S rRNA from UWB1 cells with or without ectopic WT or S1655A BRCA1 BRCT expression were quantified using RT-qPCR. Y-axis represents fold difference relative to the UWB1 cells transfected with vector alone. (D and E) Intact 5.8S rRNA (D) and ACTB (E) post RNase III treatment normalized to total 5.8S rRNA from UWB1 cells with or without ectopic expression of WT or S1655A BRCA1 BRCT were quantified using RT-qPCR. Y-axis represents fold difference relative to UWB1 cells transfected with vector alone. (F) Intact 5.8S rRNA post RNase III treatment normalized to total 5.8S rRNA from HEK293T cells transfected with control or BRCA1 siRNA was quantified using RT-qPCR. Y-axis represents fold difference relative to control siRNA. (G and H) The amounts of ds-5.8SRNA (G) and ds-ACTB mRNA (H) immunopurified using J2 dsRNA antibody normalized to input RNA from UWB1 cells with or without exogenously expressed BRCA1 BRCT fragment were quantified using RT-qPCR. Y-axis represents fold difference relative to the UWB1 cells transfected with vector alone.