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. 2022 Dec 9;119(50):e2217542119. doi: 10.1073/pnas.2217542119

Fig. 5.

Fig. 5.

as-rRNA expression suppresses ribosomal R-loops induced by CX5461. (A) Quantification of as-5.8S rRNA levels normalized to GAPDH mRNA by RT-qPCR in HEK293T cells with or without treatment with RNAPI inhibitor CX5461. Y-axis represents fold difference relative to HEK293T cells without CX5461. (B) Representative images showing immunofluorescent staining of R-loops (green) using S9.6 antibody in HEK293T cells with or without treatment with CX5461 and ectopic expression of as-5.8S rRNA. DAPI is used to show nuclei. (C and D) DRIP analysis using S9.6 antibody to measure RNase H-sensitive R-loop levels at 5.8S rDNA loci (C) and ACTB EX3 region (D) normalized to the input DNA in HEK293T cells with or without CX5461 treatment and as-5.8S rRNA overexpression. Y-axis represents fold difference relative to HEK293T cells without CX5461 or as-5.8S rRNA overexpression.