Skip to main content
. 2022 Dec 9;119(50):e2217542119. doi: 10.1073/pnas.2217542119

Fig. 6.

Fig. 6.

BRCA1-mediated as-rRNA expression negatively regulates rRNA processing. (A, Left) Schematic diagram of pre-rRNA and its processing into 18S, 5.8S, and 28S rRNA. (Right) Representative images showing northern blot analysis of the RNA samples prepared from UWB1 cells with or without ectopic BRCA1 expression using a fluorescent oligo probe complementary to either 5.8S, 18S, 28S, or ITS1 sequence. (B) Schematic diagram of 5.8S rRNA processing (Left). (Right) Representative images showing northern blot analysis of RNA samples prepared from either UWB1 cells (lanes 1 and 2) or HEK293T cells (lanes 3 and 4) with or without ectopic as-5.8S expression using an oligo probe complementary to 5.8S rRNA. (C) Quantification of as-5.8S rRNA levels normalized to ACTB mRNA by RT-qPCR in UWB1 and HEK293T cells. Y-axis represents fold difference relative to UWB1 cells.