Fig. 4.

BPA-induced changes in chromatin accessibility in oocytes, and 5mC DNA and m⁶A RNA methylation in sperm. (A) ATAC-seq sites differentially accessible between oocytes of BPA-F2 and CTL-F2 females. A subset of these sites is located in several introns of Fto and Site 3 coincides with Site 1 present in intron 8 in sperm. (B) Methylation levels at the CpG located in position 2 of the CTCF motif present in Site 1 obtained from WGBS-seq in sperm of CTL, BPA-F3, and BPA-F6. (C) Methylation levels at the same CpG obtained from bisulfite sequencing. (D) RNAs whose m⁶A levels are altered in BPA-F3 sperm with respect to control. (E) Global RNA levels are not different between sperm of BPA-F3 and control mice. (F) Levels of m⁶A at sites located in RNAs are lower in sperm from BPA-F3 mice with respect to control. (G) RNA-seq and m⁶A levels in the Fto distal CRE. This putative eRNA is present in the fraction of chromatin-associated RNA tightly bound to chromatin (SI Appendix, Fig. S4).