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. 2023 Feb 3;17(2):e0011095. doi: 10.1371/journal.pntd.0011095

Fig 3. Sialokinin knockout mosquito bites influence B cell populations in humanized mice.

Fig 3

Hu-NSG mice were subjected to four bites each from sialokinin knockout mosquitoes and were euthanized 7 days postbite. Skin (A), blood (B), spleen (C), and bone marrow (D) were collected and changes in B cell populations were assessed via flow cytometry. Mosquito Bite indicates mice bitten by wild-type mosquitoes, and Unbitten indicates mice that were not bitten by mosquitoes. Data are represented as the mean percentage of the cell population out of total human CD45+ cells. Error bars represent 1 SEM. Statistical significance was determined using two-way ANOVA followed by multiple comparison t-tests using the Holm-Sidak correction. The threshold for significance was p<0.05. Asterisks indicate: * = p<0.05; ** = p<0.01; **** = p<0.001. Cell markers used to describe populations: B cells: CD45+, CD3-, CD19+; Activated B cells: CD3-, CD19+, CD80+, CD86+; Activated and proliferating B cells: CD3-, CD19+, CD80+, CD86+, Ki67+. Abbreviations: ns = not significant.