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. 2023 Feb 3;18(2):e0270965. doi: 10.1371/journal.pone.0270965

Fig 3. Sequence alignment and active site analysis of rhodopsin.

Fig 3

(A) Alignment of human rhodopsin with remotely homologous coral proteins, only showing the positions of those amino acids that are in proximity to the ligand in the opsin family (ref). TheBallesteros-Weinstein numbering scheme is used to enable comparison to other members of the GPCR family. Positions are colored according to the degree of similarity: White foreground/black background, 100%; white foreground/grey background, > 80%, black foreground/grey background, > 60%. (B) Active site of bovine rhodopsin crystal structure 1L9H (>99% homologous to human rhodopsin) showing the residues involved in the active site shown in (A). (C) Active site showing the interaction of the ligand and vitamin A derivative, retinal, which makes rhodopsin light-sensitive, with Lys296 and Glu113 (also labeled in A,B).