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. 2023 Jan 24;12:e81923. doi: 10.7554/eLife.81923

Figure 9. NU7441 abrogates RAD51 in G1 induced by losing p300 activity.

Figure 9.

(A–B) Percentage of U2OS treated with DMSO or NU7441 (1 μM) in G1 that RAD51 staining after zeocin treatment (10 µg/mL, 10 min) as determined by (A) cyclin E staining or (B) flow cytometry. (C–D) Percentage of human foreskin keratinocyte (HFK) cells treated with DMSO or NU7441 in G1 that RAD51 staining after zeocin treatment determined by (C) cyclin E staining or (D) flow cytometry. (E–F) Percentage of HFK cells treated with DMSO, CCS1477 (1 μM), or NU7441 in G1 that RAD51 staining after zeocin treatment determined by (E) cyclin E staining or (F) flow cytometry. All values are represented as mean ± standard error. The statistical significance of differences between treatments were determined using Student’s t-test. p-Values indicate significant difference between DMSO and NU7441 with same cell line. At least 150 cells were counted over three independent experiments for microscopy. Twenty thousand cells were counted for each of three independent flow cytometry experiments.