Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | HFK | This paper | Derived from neonatal foreskins | |
| Cell line (Homo sapiens) | N/TERT HFK | Michael Underbrink (PMID:18256157) | N/TERT immortalized HFK | |
| Cell line (Homo sapiens) | U2OS | PMID:10541549 | Cell line used to measure HR frequency | |
| Recombinant DNA reagent | Alt-EJ reporter | Addgene | #113619 | Alt-EJ reporter using 4 nt microhomology |
| Recombinant DNA reagent | Alt-EJ reporter (5’ end) | Addgene | #113620 | sgRNA/CAS9 to induce the 5’ end DSB |
| Recombinant DNA reagent | Alt-EJ reporter (terminal) | Addgene |
#113625 #113625 |
sgRNA/CAS9 to induce the DSB at the edge of the microhomology |
| Recombinant DNA reagent | Alt-EJ reporter (imbedded) | Addgene | #113626 | sgRNA/CAS9 to induce the DSB 8 nt upstream of the microhomology |
| Antibody | Anti-RAD51 (Mouse monoclonal) |
Abcam | ab1837 | IF (1:200) |
| Antibody | Anti-cyclin E (Rabbit monoclonal) | Cell Signaling | 4132S | IF (1:200) |
| Antibody | Anti-pH2AX S139 (Rabbit monoclonal) | Cell Signaling | 9718S | IF (1:200) |
| Antibody | Anti-cyclin A (Mouse monoclonal) | Abcam | ab39 | IF (1:200) |
| Antibody | Alexa Fluor 594 (Goat polyclonal) | Thermo Fisher Scientific | A11012 | IF (1:500) |
| Antibody | Alexa Fluor 488 (Goat polyclonal) | Thermo Fisher Scientific | A11001 | IF (1:500) |
| Chemical compound, drug | CCS1477 | Chemietek | CT-CCS1477 | P300 inhibitor |
| Chemical compound, drug | NU7441 | Selleckchem | S2638 | DNA-PKcs inhibitor |
| Chemical compound, drug | Zeocin | Alfa Aesar | J67140-XF | Used to induce DSBs |
| Chemical compound, drug | ART558 | MedChem Express | HY-141520 | Pol Theta inhibitor |
| Chemical compound, drug | DAPI stain | Invitrogen | D1306 | IF (10 µM) |
| Chemical compound, drug | NUCLEAR-ID Red | Enzo Life Science | ENZ-52406 | Flow cytometry (1:1000) |
| Software, algorithm | ImageJ | ImageJ (https://imagej.nih.gov/ij/) | Version 2.3.0 | |
| Software, algorithm | GraphPad Prism | GraphPad Prism (https://graphpad.com) | Version 9.0.0 |