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Ure2p is aggregated in [URE3] cells. A Ure2p-GFP fusion was expressed from a single-copy plasmid with the URE2 promoter, and the distribution of fluorescence was examined in [ure-o] (wild-type), [URE3], and guanidine-cured strains. Aggregation was detected specifically in [URE3] strains (37).
