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Amyloid is formed in vitro by Ure2p. (a) Filaments (45 Å) formed by Ure2p1–65. (b) Ure2p1–65/Ure2p 1:1 cofilaments 200 Å in diameter. (c) Ure2p1–65/Ure2p cofilaments digested with proteinase K, leaving the prion domain protease resistant in the form of narrow filaments. (d) Filaments (280 to 400 Å) of native Ure2p formed by seeding with Ure2p1–65/Ure2p cofilaments. Modified from reference 90 with permission of the publisher.
