Table 2.

Minimum panels for cell characterization by flow cytometry of Waldenström’s macroglobulinemia at diagnosis.

Tube	FITC	PE	PERCP-Cy5.5	PE-Cy7	APC	APC-H7/APC-Cy7	PacB/BV450	PacO/OC515
1	SIgM (5 µl)	CD25 (10 µl)	CD22 (10 µl)	CD19 (5 µl)	CD27 (5 µl)	CD38 (3 µl)	CD20 (5 µl)	CD45 (5 µl)
2	CyIgM (5 µl)	CyL (5 µl)	CD5 (10 µl)	CD19 (5 µl)	CyK (5 µl)	CD38 (3 µl)	CD20 (5 µl)	CD45 (5 µl)

Adapted from Puig et al. [33].

FICT fluorescein isothiocyanate, PE phycoerythrin, PERCP peridinin-chlorophyll-protein complex, Cy5.5 Cyanine 5, Cy7 Cyanine 7, APC allophycocyanin, PacB Pacific Blue, BV450 Brilliant Violet 450, PacO Pacific Orange, OC515 Orange Cytognos 51.