Figure 1.

The maps of target lymphocytes and DPT cells by flow cytometry.

A: Differentiation of lymphocytes by anti-CD45 peridinin chlorophyll protein (PerCP) and side scatter (SSC). Lymphocytes are included in the polygonal check box. B: Differentiation of T lymphocytes by anti-CD3 fluorescein isothiocyanate (FITC). T lymphocytes were included in the rectangular check box. C: Differentiation of CD4⁺CD8⁺ double positive T lymphocytes by anti-CD8 phycoerythrin (PE) and anti-CD4 allophycocyanin (APC). The cruciform check box was used to divide the T4, DPT, DNP, and T8 lymphocytes. P1: all cells; P2: karyocytes; T4: CD3⁺CD4⁺CD8⁻ T lymphocytes; T8: CD3⁺CD4⁻CD8⁺ T lymphocytes; Ly: lymphocytes; DP: CD4⁺CD8⁺ double positive T lymphocytes; DN: CD4⁻CD8⁻ double negative T lymphocytes.