TABLE 4.
Archaeal Hsp70(DnaK) amino acid sequences deduced from cloned genes: motifs
| Motifa | Function | Reference(s) | Synonyma |
|---|---|---|---|
| Phosphate 1 | ATPase; nucleotide binding | 23 | Hsp70 family sig. (ATP α-phosphate) |
| Connect 1 | 23 | Hsp70 family sig. (6 aa) | |
| Phosphate 2 | 23 | Hsp70 family sig. | |
| Adenosine | 23 | None | |
| Connect 2 | 23 | None | |
| DnaK loop [N-29,b (A,S)-30, E-31, G-32, R-34, E-369] | GrpE binding | 28 | None |
| Residue E-171 | Hinge | 29 | None |
| T-12, T-13, D-367 | Interdomain communication | 265, 268 | None |
| Leucine zipper | Oligomerization (?) | 180a | None |
| Hsp70 family sigs. (6 & 7 aa) | ? | 239 | None |
| EEDKKRRERb (archaea, Gram+; not in Gram−) | ? | 142 | Hypercharge run |
| NLS (eukaryotic ct) | Nuclear localization signal | 239 | None |
| Eukaryotic (ct; ER) Hsp70 sig. | ? | 239 | None |
| EEVDb (eukaryotic Hsp70 COOH end) | DnaJ binding; regulatory | 81 | None |
| GDAWVb (mitochondria & alpha, beta, gamma proteobacteria) | ? | 91 | mt sig. Box A |
| Reference | Motif presentc
|
|||||
|---|---|---|---|---|---|---|
| M. mazei S-6 (182) | M. thermophila TM-1 (126) | M. thermoautotrophicum ΔH | T. acidophilum (111) | H. cutirubrum (111) | H. marismortui (110) | |
| 8 | Yes | Yes | Yes | Yes | Yes | Yes |
| 239 | Yes | Yes | Yes | Yes | Yes | Yes |
| 8 | Yes | Yes | Yes | Yes | Yes | Yes |
| NA | Yes | Yes | Yes | Yes | Yes | Yes |
| NA | Yes | Yes | Yes | Yes | Yes | Yes |
| NA | N-29 | N-29 | N-31 | N-31 | ||
| A-30 | A-30 | A-33 | S-30 | |||
| E-31 | E-31 | E-34 | E-31 | E-33 | E-33 | |
| G-32 | G-32 | G-35 | G-32 | G-34 | G-34 | |
| R-34 | R-34 | R-36 | ||||
| E-376 | E-376 | E-364 | E-362 | E-375 | E-375 | |
| NA | E-183 | E-183 | E-170 | E-168 | E-160 | E-162 |
| D-171 | D-171 | D-169 | D-174 | D-171 | D-171 | |
| NA | T-11 | T-11 | T-14 | T-11 | T-13 | T-13 |
| T-12 | T-12 | S-15 | S-12 | T-14 | T-14 | |
| D-367 | D-367 | D-367 | D-365 | D-366 | D-366 | |
| NA | Yes | Yes | Yes | Yes | Yes | Yes |
| NA | Yes | Yes | Yes | Yes | Yes | Yes |
| 142 | Yes | Yes | Yes | Yes | Yes | Yes |
| NA | No | No | No | No | No | No |
| NA | No | No | No | No | No | No |
| NA | E-611 | No | No | No | E-624 | E-626 |
| V-612 | D-625 | D-627 | ||||
| V-613 | V-626 | V-628 | ||||
| D-614 | D-627 | E-629 | ||||
| 91 | No | No | No | No | No | No |
a
Abbreviations: NA, not applicable; sig. or sigs., signature(s); aa, amino acid(s); NLS, nuclear localization signal; ct, cytosol; ER, endoplasmic reticulum; mt, mitochondria.
b
Amino acid single-letter symbols (followed by position number when pertinent).
c
References are given in parentheses. Accession numbers are the same as in Table 3.