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. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: Nat Chem Biol. 2022 Oct 27;19(2):230–238. doi: 10.1038/s41589-022-01168-3

Extended Data Fig. 2. De novo CoQ10 biosynthesis measurements.

Extended Data Fig. 2

a, Schematic outlining the experimental plan to measure de novo CoQ production. b, De novo production of 13C6-PPHB10 in WT, COQ8AKO, COQ8BKO, and COQ8A/BDKO HAP1 cells after treatment with 10 μM 13C6-4-HB and either DMSO or 20 μM UNC-CA157 (grey=DMSO, red=UNC-CA157, n=3 independent samples from one experiment, mean ± SD) (two sided students t-test, no adjustment for multiple comparisons). c, Unlabeled PPHB10 levels in WT, COQ8AKO, COQ8BKO, and COQ8A/BDKO HAP1 cells after treatment with 10 μM 13C6-4-HB and either DMSO or 20 μM UNC-CA157 (grey=DMSO, red=UNC-CA157, n=3 independent samples from one experiment, mean ± SD) (two sided students t-test, no adjustment for multiple comparisons). d, Unlabeled CoQ10 levels in WT, COQ8AKO, COQ8BKO, and COQ8A/BDKO HAP1 cells after treatment with 10 μM 13C6-4-HB and either DMSO or 20 μM UNC-CA157 (grey=DMSO, red=UNC-CA157, n=3 independent samples from one experiment, mean ± SD) (two sided students t-test, no adjustment for multiple comparisons).