KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| mouse anti-S100β | Sigma-Aldrich | Cat# S2532; RRID:AB_477499 |
| rabbit anti-S100β | Abcam | Cat# ab41548; RRID: AB_956280 |
| chicken anti-S100β | Synaptic Systems | Cat# 287006; RRID:AB_2713986 |
| mouse anti-NeuN (clone A60) | Millipore | Cat# MAB377; RRID: AB_2298772 |
| chicken anti-GFP | Abcam | Cat# ab13970; RRID: AB_300798 |
| rabbit anti-RFP | Rockland | Cat# 600–401-379; RRID: AB_2209751 |
| mouse anti-HA | BioLegend | Cat# 901514, RRID:AB_2565336 |
| guinea pig anti-NeuN | Synaptic Systems | Cat# 266 004, RRID:AB_2619988 |
| rabbit anti-NeuN | Cell Signaling Technology | Cat# 12943, RRID:AB_2630395 |
| rabbit anti-Darpp32 | Abcam | Cat# ab40801, RRID:AB_731843 |
| mouse anti-mHTT (clone mEM48) | Millipore | Cat# MAB5374, RRID:AB_10055116 |
| mouse anti-mHTT (clone 2B4) | Millipore | Cat# MAB5492, RRID:AB_347723 |
| Alexa Fluor 488 goat anti-chicken | Molecular Probes | Cat# A11039; RRID: AB_2534096 |
| Alexa Fluor 488 goat anti-rabbit | Molecular Probes | Cat# A11008; RRID: AB_143165 |
| Alexa Fluor 546 goat anti-mouse | Molecular Probes | Cat# A11003; RRID: AB_2534071 |
| Alexa Fluor 546 goat anti-chicken | Molecular Probes | Cat# A11040; RRID: AB_2534097 |
| Alexa Fluor 594 goat anti-rabbit | Molecular Probes | Cat# R37117; RRID: AB_2556545 |
| Alexa Fluor 647 goat anti-rabbit | Molecular Probes | Cat# A21245; RRID: AB_ 2535812 |
| Bacterial and Virus Strains | ||
| AAV2/5 GfaABC1D Rpl22HA | Yu et al., 2018 | Addgene Vectors #111811RRID:Addgene_111811 |
| AAV5 hSyn1 Rpl22HA | This paper | Addgene Vectors #177685RRID:Addgene_177685 |
| AAV2/5 GfaABC1D ZFPDeltaDBD-HA P2A tdTomato | Diaz-Castro et al, 2019 | See note 1 |
| AAV2/5 GfaABC1D ZFP30645-HA P2A tdTomato | Diaz-Castro et al, 2019 | See note 1 |
| AAV PHP.eB GfaABC1D ZFPDeltaDBD-HA P2A tdTomato | This paper | See note 1 |
| AAV PHP.eB GfaABC1D ZFP30645-HA P2A tdTomato | This paper | See note 1 |
| AAV5 hSyn1 ZFPDeltaDBD-HA P2A tdTtomato | This paper | See note 1 |
| AAV5 hSyn1 ZFP30645-HA P2A tdTomato | This paper | See note 1 |
| AAV PHP.eB hSyn1 ZFPDeltaDBD-HA P2A tdTomato | This paper | See note 1 |
| AAV PHP.eB hSyn1 ZFP30645-HA P2A tdTomato | This paper | See note 1 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Formalin, Buffered, 10% | Fisher Chemical | Cat# SF100–20 |
| Pronase | Sigma-Aldrich | Cat# P6911 |
| Fetal bovine serum | Thermo Fisher Scientific | Cat# 10437028 |
| BSA | Sigma-Aldrich | Cat# A8806 |
| Phosphate buffered saline | Sigma-Aldrich | Cat# P4417–100TAB |
| Formic acid | Sigma-Aldrich | Cat# 695076 |
| Experimental Models: Organisms/Strains | ||
| Mouse: B6CBA-Tg(HDexon1)62Gpb/3J | Jackson Laboratory | Stock# 006494; RRID:IMSR_JAX:006494 |
| Mouse: B6J.129S1-Htttm1Mfc/190ChdiJ | Jackson Laboratory | Stock# 027410; RRID:IMSR_JAX:027410 |
| Mouse: C57Bl/6NJ | Jackson Laboratory | Stock# 005304; RRID:IMSR_JAX:005304 |
| Mouse: C57Bl/6NTac inbred mice | Taconic | Stock# B6; RRID:IMSR_TAC:b6 |
| Oligonucleotides | ||
| C57insF (5’-CTGGAATTAGGAATGGTTGTGTG-3’) | Smith et al., 2021 | N/A |
| C57insR (5’-TTGTTCTTTACTTTCCTTGTTTGAGAAT-3’) | Smith et al., 2021 | N/A |
| CBAinsF2 (5’-TAGGAATGGTTGTAAACCAGG-3’) | Smith et al., 2021 | N/A |
| CBAinsR2 (5’-CCTCCATTGGGAACTGCTG-3’) | Smith et al., 2021 | N/A |
| Mutant Htt forward (5’-CGCAGGCTGCAGGGTTAC-3’) | Zeitler et al., 2019 | N/A |
| Mutant Htt reverse (5’-GCTGCACCGACCGTGAGT-3’) | Zeitler et al., 2019 | N/A |
| Mutant Htt prove (5’-CAGCTCCCTGTCCCGGCGG-3’) | Zeitler et al., 2019 | N/A |
| Atp5b (Mm.PT.53a.17279462) | Integrated DNA Technologies, Inc. | N/A |
| Htt (Mm.PT.58.6953479) | Integrated DNA Technologies, Inc. | N/A |
| Software and Algorithms | ||
| OriginPro 2016 | Origin Lab Corporation | RRID:SCR_015636 |
| pCLAMP10.4 | Molecular Devices | RRID:SCR_011323 |
| ClampFit10.4 | Molecular Devices | N/A |
| Fluoview FV10-ASW | Olympus | N/A |
| Fluoview FV31S-SW | Olympus | N/A |
| ImageJ v1.51h | NIH | RRID:SCR_003070 |
| ANY-maze | Stoelting Co. | RRID:SCR_014289 |
| MaxQuant 2.0.3.0 | https://maxquant.org | RRID:SCR_014485 |
| Cytoscape 3.8.2 | https://cytoscape.org | RRID:SCR_003032 |
| CorelDraw X7 | Corel Corporation | RRID:SCR_014235 |
| STRING database | https://string-db.org | RRID:SCR_005223 |
| MSstats Bioconductor package | https://msstats.org | RRID:SCR_014353 |
| Bioconductor | http://www.bionconductor.org | RRID:SCR_006442 |
| Deposited Data | ||
| Astrocyte, neuron and bulk RNA-seq: | This paper | GEO: GSE189647GEO: GSE211891 |
| Bulk tissue proteomics | This paper | PRIDE: PXD030021 |
| Statistical tests used in the figures | This paper | Excel file S7 |
Note 1:
The vectors listed incorporating Sangamo Biosciences / Takeda Inc mHTT zinc finger repressor proteins ZFP-30645 and ZFP-DBD were made by the Khakh lab. The original plasmids were obtained via CHDI Foundation under the terms of a Use Agreement by and between CHDI Foundation and Sangamo Biosciences. Requests for these plasmids should be made directly to CHDI Foundation (info@CHDIFoundation.org) with ZFP in the subject line.