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. Author manuscript; available in PMC: 2024 Feb 15.
Published in final edited form as: J Immunol. 2023 Feb 15;210(4):377–388. doi: 10.4049/jimmunol.2200325

Figure 2. The inhibition of glycolysis does not impair cognate AM14 AFC differentiation in lymphocyte-deficient mice.

Figure 2.

AM14 B cells were transferred into BALB/c.Rag mice immunized with PL2-3 or R848, and treated or not with 2DG for 7 d. A. Absolute numbers of splenocytes. B. 4–44 IgM ELISPOTs. C. Serum 4–44 IgM. D. Absolute AM14 B cell numbers. E. Frequency of proliferating Ki-67+ AM14 B cells. F. Frequency of IgDlowKi-67+ among AM14 B cells. Frequency (G) and number (H) of PBs among AM14 B cells. K. Ex vivo 2NBDG uptake in total AM14 cells (left) or AM14 PBs (right). L. Bodipy uptake in total AM14 cells (left) or AM14 PBs (right). Data was pooled from 2 – 4 experiments. Each point represents a mouse and graphs show means and SEM. Statistics were calculated with 1-way ANOVA with multiple comparison tests. *P < 0.05; **P < 0.01. ***P < 0.001.