Figure 7. Glycolysis is dispensable for foreign antigen-specific CD4+ T cells in response to immunization.

A – L. CD45.1⁺ OT-II T cells were transferred into B6 mice immunized with NP-OVA in alum, and treated or not with 2DG for 7 d. A. Numbers of OT-II cells. B. Frequency of proliferating OT-II cells. C. Frequency of Tem cells among OT-II cells. D. Frequency of Tfh cells among OT-II cells. E. Frequency of proliferating OT-II Tfh cells. Frequency of NP⁺ GC B (F) and NP⁺ PC (G) cells. Frequency of CS in NP⁺ GC B (H) and PC (I) cells. J. Serum levels of anti-NP₂₅ IgG1. K. Proliferation in endogenous total CD4⁺ or Tfh cells. L. Frequency of Tem and Tfh cells among endogenous B6 CD4⁺ T cells. M – O. DO11-10 T cells were transferred into AM14 mice immunized with NP-OVA in alum, and treated or not with 2DG for 7 d. M. Numbers of splenocytes in AM14 recipient mice. Frequency of total DO11.10 T cells among splenocytes (N), as well as proliferating (left), CD44⁺ (middle) and Tfh (right) cells among DO11.10 T cells (O). (A – L) data were pooled from two experiments. Each point represents a mouse and graphs show means and SEM. Statistics were calculated with 1-way ANOVA with multiple comparison tests. *P < 0.05; **P < 0.01. ***P < 0.001.